ll also be introduced in this chapter.

logy question

man spectroscopy technology [Gardiner, 1989] was invented in

sed on the work of an Indian scientist Chandrasekhara Venkata

who won the Nobel prize in physics along with several other

in 1930 for his invention of the Raman spectroscopy technology.

c working principle of the Raman spectroscopy technology is to

molecules so that inelastically scattered light caused by these

molecules will demonstrate some variable shift of energy for

photons.

d on the Raman spectroscopy technology, two sets of spectra were

d [Lau, et al., 2012]. They were 11 replicates of the J774.1

ages and nine replicates of the Salmonella enterica serovar

rium SL1344 bacteria grown at four-time settings using an

nt named by a Renishaw RM1000 Raman microscope. The

were generated based on a 785-nm laser with 300-mW excitation.

pment was named as RENISHAW from the Wooton-Under-Edge

K. The four-time settings for growing bacterial samples were 30

90 minutes, 150 minutes and 360 minutes. Each had nine

s. The questions of how to extract peaks, discover chemicals from

ctra and what the discrimination power these spectra profiles

uire investigation.

duction of baseline estimation approaches

chemicals or molecules have different weights, sizes and

s, they may generate different light spectra during vibration.

sitions in a whole spectrum will then be treated as the molecule

nts in a sample. At different positions, different chemicals will

ferent intensities, which are seen as the peak heights.

ver, the spectrum generated by the spectroscopy technology is

mposed of a complex mixture between the signals and a baseline.